Mouse Heart

Product Information

The hearts worked against an afterload reservoir at a height equivalent to 50 mmHg, and heart rate was controlled by electrical pacing of the right atrium. in which the whole mouse embryo is immobilized by inserting the extraembryonic region in a holder ( Figure 4C). cre/+;Rosa26Rtdtomato +/- line, instead show strong tdtomato level in both the FHF and SHF ( Figure 3—figure supplement 3A).

Like a good Agatha Christie, the murder’s do not stop at the first, and I sensed each tragedy before they happened – such is the brilliance of Hitchcock’s storytelling. and specific mechanisms affecting these pathways could be operating during the formation the HT, whereby the differentiation pathways could be temporally restrained. These studies and our observations, however, cannot discriminate whether this lineage allocation results from intrinsic differences between these lineages or it is due to their exposure to position-specific environments, especially as in our studies sister cells remain close neighbors. C) Inset from A (Red frame); increase of the tdtomato intensity in the splanchnic mesoderm over time. and other views suggest that the heart would form by a continuous differentiation process from a single population of cardiac precursors and only timing of recruitment would distinguish cells of the FHF and SHF ( Abu-Issa et al.These 2D image stacks can be readily resliced in any arbitrary imaging plane, and using these 2D image stacks, 3D reconstructions of the specimen can be generated with ease. cre/+; Rosa26Rtdtomato +/- embryos, tdtomato labeling is also observed in the endocardium and endothelial cells ( Stanley et al. From transversal to open HT (5–7 hr) and from the open HT to HT (2–3 hr) the GFP signal remains stable ( Figure 5—figure supplement 1B,B’, C,C’ and Video 12). cTnnT+ cells are initially columnar epithelial cells and show apical localization of the tight junction component zona-occluden-1 (ZO-1) ( Figure 1—figure supplement 3B,B’). The live imaging, however, did not allow to unambiguously identify all cells located deep inside the live tissue at the final stages recorded.

is also transiently expressed in FHF precursors and must therefore be considered as widespread cardiac progenitor marker instead ( Cai et al.This is consistent with previous studies in mouse, chick and human models showing that proliferation drops in the differentiated myocardium of the forming HT, while proliferation remains high in the splanchnic mesoderm ( van den Berg et al. By combining various genetic tracing tools, we labeled progenitor and differentiated cardiomyocytes and performed 3D cell tracking over time combined with 3D reconstruction of the HT at multiple stages. No solid domains containing double-labeled cells were detected, indicating that progenitors located in the SHF did not undergo differentiation in the boundary zone from cc to open HT stage ( Figure 8D). What’s more, we realise that while she’s beloved by Mrs Hawkin, Walter, Kwadwo and Adam, her confidence and skills might be underestimated by others. embryos, fluorophore signal present in the endothelium, endocardium and endoderm cells was manually masked prior to segmentation ( Figure 1A, Figure 1—figure supplement 1, Figure 2B, Figure 2—figure supplement 2A,A’ and, Figure 6A’’).