Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate.

Iris Dyes | Cyanine Technologies". Archived from the original on 2015-01-26 . Retrieved 2015-01-26. The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column. The additive effect of multiple Gs or Cs in a row is not immediately apparent, with GGGGG ranking #31 and #21 (0.71 and 0.64 relative intensity) and CCCCC ranking #1009 and #1021 (0.37 and 0.25 relative intensity) for Cy3 and Cy5, respectively. The top-ranking GAAAA motif identified in 5ʹ-Cy3 oligonucleotides here ranks #334 (0.58 relative fluorescence), further illustrating the significant differences between 5ʹ and 3ʹ labeling.

Kvach, Maksim V.; Ustinov, Alexey V.; Stepanova, Irina A.; Malakhov, Andrei D.; Skorobogatyi, Mikhail V.; Shmanai, Vadim V.; Korshun, Vladimir A. (2008). "A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules". European Journal of Organic Chemistry. 2008 (12): 2107–2117. doi: 10.1002/ejoc.200701190. ISSN 1099-0690.

Sulfo–Cyanine dyes bear one or two sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher water solubility. [8] PEGylation is another modification that confers hydrophilicity, not only to the dye but also to the labeled conjugate.Iqbal, A., Wang, L., Thompson, K. C., Lilley, D. M. J. & Norman, D. G. The structure of cyanine 5 terminally attached to double-stranded DNA: Implications for FRET studies. Biochemistry 47, 7857–7862 (2008). Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. The excitation source will now appear on the graph display as a thin vertical line in a color associated with its location on the spectrum. The amount of Cy5.5 required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of Cy5.5 to protein is about 10. Schneider, T. D. & Stephens, R. M. Sequence logos—a new way to display consensus sequences. Nucleic Acids Res. 18, 6097–6100 (1990).